How to reconstitute research peptides.
A short, lab-grade walk-through of how to mix lyophilized research peptides with bacteriostatic water — including common ratios, storage, and pitfalls. For laboratory research use only.
Gather your supplies
- Your lyophilized peptide vial (kept refrigerated until use).
- Bacteriostatic water — sterile water containing 0.9% benzyl alcohol.
- A sterile 1 mL or 3 mL syringe with a fine-gauge needle.
- Alcohol swabs and a clean, flat work surface.
Pick a reconstitution volume
Volume is a tradeoff between draw precision and convenience. Common ratios for research vials:
| 2 mg vial | 1–2 mL water |
| 5 mg vial | 1–3 mL water |
| 10 mg vial | 2–3 mL water |
| 20 mg vial | 2–5 mL water |
Need exact numbers for your dose? Use the peptide dosing calculator — it solves for concentration, draw volume, and syringe units.
Reconstitute slowly
- Wipe both vial stoppers with an alcohol swab and let them air-dry.
- Draw your chosen water volume into the syringe.
- Inject the water slowly down the inside glass wall of the peptide vial. Do not aim the stream at the powder.
- Once added, swirl the vial gently between your fingers until the peptide fully dissolves. Do not shake — agitation can denature the peptide chain.
Label and store
Reconstituted peptides should be stored at 2–8 °C and protected from light. Label the vial with the date, water volume, and resulting concentration (mg/mL). Most reconstituted peptides remain stable for 2–4 weeks refrigerated; check your CoA for compound-specific guidance.
